1ST8

Crystal structure of fructan 1-exohydrolase IIa from Cichorium intybus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	100
Detector technology	CCD
Collection date	2002-05-23
Detector	MARRESEARCH
Wavelength(s)	0.81100
Spacegroup name	P 41 21 2
Unit cell lengths	139.830, 139.830, 181.940
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.600 - 2.350
R-factor	0.183
Rwork	0.183
R-free	0.20000
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	1.400
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.390
High resolution limit [Å]	2.350	2.350
Rmerge	0.075
Number of reflections	69991
Completeness [%]	84.0	98.7
Redundancy	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	277	sodium potassium phosphate, potassium phosphate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K