1ST3

THE CRYSTAL STRUCTURE OF THE BACILLUS LENTUS ALKALINE PROTEASE, SUBTILISIN BL, AT 1.4 ANGSTROMS RESOLUTION

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	53.093, 61.490, 75.610
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.600 - 1.400
R-factor	0.19
Rwork	0.190
RMSD bond length	0.015
RMSD bond angle	2.560
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000 *
High resolution limit [Å]	1.360 *	1.360 *
Total number of observations	171751 *
Number of reflections	49154 *
Completeness [%]	92.7 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	PEG3350
2	1	drop	citrate	13-16 (%(w/v))