1SSY

Crystal structure of phage T4 lysozyme mutant G28A/I29A/G30A/C54T/C97A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.54
Spacegroup name	C 1 2 1
Unit cell lengths	123.020, 53.720, 60.490
Unit cell angles	90.00, 102.32, 90.00

Refinement procedure

Resolution	18.520 - 2.400
R-factor	0.175
Rwork	0.175
R-free	0.23600
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.200
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.400
Number of reflections	13604

Crystallization Conditions

crystal ID	method	pH	temperature	details
1