1SSQ

Serine Acetyltransferase- Complex with Cysteine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	125
Detector technology	IMAGE PLATE
Collection date	2003-01-07
Detector	RIGAKU RAXIS IV
Spacegroup name	H 3
Unit cell lengths	97.524, 97.524, 108.457
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	22.900 - 1.850
R-factor	0.201
Rwork	0.198
R-free	0.26100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ssm
RMSD bond length	0.019
RMSD bond angle	1.900
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	EPMR
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	22.900	1.970
High resolution limit [Å]	1.850	1.850
Rmerge	0.083	0.218
Number of reflections	32779
Completeness [%]	99.8	99.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.4	298	100 mM MES, 20-40 mM MgCl2, 2% (v/v) 2-propanol, 5 mM DTT, pH 5.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K