1SS8
GroEL
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 178.380, 204.980, 280.980 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.700 |
| R-factor | 0.216 |
| Rwork | 0.215 |
| R-free | 0.24900 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.027 |
| RMSD bond angle | 2.043 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (refmac_5.1.19) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.015 |
| High resolution limit [Å] | 2.700 |
| Number of reflections | 117825 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION, RECRYSTALLIZATION | 8 | 298 | PEG 8000, 1.72 M ammonium slufate, 4 mM CaCl2, 100mM Tris-acetate , pH 8.0, EVAPORATION, RECRYSTALLIZATION, temperature 298K |
