1SRV
THERMUS THERMOPHILUS GROEL (HSP60 CLASS) FRAGMENT (APICAL DOMAIN) COMPRISING RESIDUES 192-336
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-11-15 |
| Detector | RIGAKU RAXIS |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 63.470, 65.960, 75.030 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.700 |
| R-factor | 0.193 |
| Rwork | 0.199 |
| R-free | 0.25700 |
| Structure solution method | MAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 0.038 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CCP4 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.058 | 0.390 |
| Number of reflections | 15546 | |
| <I/σ(I)> | 17.2 | 2.2 |
| Completeness [%] | 88.0 | 46 |
| Redundancy | 3.8 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 * | pH 7.5, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 21 (mg/ml) | |
| 2 | 1 | drop | PEG6000 | 4.5 (%) | |
| 3 | 1 | drop | HEPES | 50 (mM) | |
| 4 | 1 | drop | MPD | 2.5 (%) | |
| 5 | 1 | reservoir | PEG6000 | 9 (%) | |
| 6 | 1 | reservoir | HEPES | 100 (mM) | |
| 7 | 1 | reservoir | MPD | 5 (%) |
