1SRU
Crystal structure of full length E. coli SSB protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.9879, 0.9794, 0.9792 |
| Spacegroup name | P 31 1 2 |
| Unit cell lengths | 60.850, 60.850, 348.920 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.920 - 3.300 |
| R-factor | 0.288 |
| Rwork | 0.288 |
| R-free | 0.30900 |
| Structure solution method | SAD/MAD/MR |
| RMSD bond length | 0.028 |
| RMSD bond angle | 2.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.400 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Number of reflections | 11620 | |
| Completeness [%] | 100.0 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | PEG4000, PEG200, Hepes, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
