1SRR
CRYSTAL STRUCTURE OF A PHOSPHATASE RESISTANT MUTANT OF SPORULATION RESPONSE REGULATOR SPO0F FROM BACILLUS SUBTILIS
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 90 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-01 |
| Detector | MARRESEARCH |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 101.990, 101.990, 82.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.900 |
| R-factor | 0.219 |
| Rwork | 0.219 |
| R-free | 0.27800 |
| Structure solution method | SINGLE ISOMORPHOUS AND ANOMALOUS REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 18.500 * |
| Data reduction software | DENZO |
| Phasing software | PHASES |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.070 | 0.452 |
| Total number of observations | 133198 * | |
| Number of reflections | 34309 | |
| <I/σ(I)> | 19.2 | 2.6 |
| Completeness [%] | 98.5 | 98.3 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.3 * | 277 * | Madhusudan, Z.J., (1996) Acta Cryst.D., 52, 589. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | bis-Tris | 10 (mM) | |
| 2 | 1 | drop | 50 (mM) | ||
| 3 | 1 | drop | 0.02 (%) | ||
| 4 | 1 | drop | protein | 25 (mg/ml) | |
| 5 | 1 | reservoir | PEG3350 | 6-7.5 (%) | |
| 6 | 1 | reservoir | glycerol | 10 (%) | |
| 7 | 1 | reservoir | 200 (mM) | ||
| 8 | 1 | reservoir | 100 (mM) |
