1SRN

THE REFINED CRYSTAL STRUCTURE OF A FULLY ACTIVE SEMISYNTHETIC RIBONUCLEASE AT 1.8 ANGSTROMS RESOLUTION

Experimental procedure

Spacegroup name	P 32 2 1
Unit cell lengths	67.680, 67.680, 65.030
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 1.800
R-factor	0.204
RMSD bond length	0.024
Refinement software	PROLSQ

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	10.000 *
High resolution limit [Å]	1.800 *	1.800 *
Rmerge	2.000 *
Number of reflections		4828 *
Completeness [%]		57 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	RNase1-118	0.95 (mM)
2	1	drop	RNase111-124	9.9 (mM)
3	1	reservoir		3.0 (M)
4	1	reservoir	ammonium sulfate	28 (%sat)
5	1	reservoir	ammonium acetate	0.1 (M)
6	1	reservoir	1,10-phenanthroline	5 (mM)