1SR8
Structural Genomics, 1.9A crystal structure of cobalamin biosynthesis protein (cbiD) from Archaeoglobus fulgidus
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-02-18 |
| Detector | SBC-3 |
| Wavelength(s) | 0.9795, 0.9797, 0.94656 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 79.035, 75.912, 63.032 |
| Unit cell angles | 90.00, 113.70, 90.00 |
Refinement procedure
| Resolution | 26.990 - 1.900 |
| R-factor | 0.209 |
| Rwork | 0.209 |
| R-free | 0.25800 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.200 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.048 | 0.405 |
| Number of reflections | 52336 | |
| <I/σ(I)> | 24.17 | 2.554 |
| Completeness [%] | 98.2 | 93 |
| Redundancy | 4 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 0.2M K/Na Tartr., 20% PEG3350, 0.05M Tris, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
