1SR7
Progesterone Receptor Hormone Binding Domain with Bound Mometasone Furoate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-02-15 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.112, 64.256, 70.091 |
| Unit cell angles | 90.00, 96.88, 90.00 |
Refinement procedure
| Resolution | 19.960 - 1.460 |
| R-factor | 0.1762 |
| Rwork | 0.174 |
| R-free | 0.21061 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1a28 |
| RMSD bond length | 0.043 |
| RMSD bond angle | 3.101 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | |
| High resolution limit [Å] | 1.460 | 1.460 |
| Rmerge | 0.050 | 0.220 |
| <I/σ(I)> | 19.7 | 3.5 |
| Completeness [%] | 98.5 | 98.9 |
| Redundancy | 3 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 300 | PEG 1000, Li2SO4, hepes, glycerol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
