1SQU
Structural Genomics, Crystal structure of the CheX protein from Thermotoga maritima
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-03-02 |
Detector | SBC-2 |
Wavelength(s) | 0.9795, 0.9797, 0.94656 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 55.241, 73.031, 78.341 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.150 - 2.400 |
R-factor | 0.239 |
Rwork | 0.239 |
R-free | 0.32000 |
Structure solution method | MAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.400 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.480 |
High resolution limit [Å] | 2.380 | 2.380 |
Rmerge | 0.081 | 0.580 |
Number of reflections | 12325 | |
<I/σ(I)> | 31.7 | 2.33 |
Completeness [%] | 93.4 | 91.6 |
Redundancy | 6 | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 289 | 20% PEG 3350, 10% isopropanol, 0.1M sodium Cacodylate, 15% MPD, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K |