1SQS
X-Ray Crystal Structure Protein SP1951 of Streptococcus pneumoniae. Northeast Structural Genomics Consortium Target SpR27.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-02-28 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.161, 81.994, 63.450 |
| Unit cell angles | 90.00, 94.62, 90.00 |
Refinement procedure
| Resolution | 28.760 - 1.500 |
| Rwork | 0.200 |
| R-free | 0.22000 |
| Structure solution method | SAD |
| Starting model (for MR) | None |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.000 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SnB |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.760 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.056 | 0.277 |
| Number of reflections | 154553 | |
| <I/σ(I)> | 19.74 | 3.8 |
| Completeness [%] | 96.9 | 92 |
| Redundancy | 4.1 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | Protein solution: 10mM Tris (pH 7.5), 5mM DTT. Well solution: 10% PEG 3350, 100mM Sodium Tartrate, 200mM NaCl., VAPOR DIFFUSION, HANGING DROP, temperature 277K |
