1SQM
STRUCTURE OF [R563A] LEUKOTRIENE A4 HYDROLASE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-01-22 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.96800 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 78.000, 86.850, 98.950 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 16.480 - 2.300 |
| R-factor | 0.191 |
| Rwork | 0.190 |
| R-free | 0.23000 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.445 |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 16.480 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.092 | |
| Number of reflections | 30528 | |
| Completeness [%] | 99.7 | 99.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIQUID DIFFUSION | 8 | 298 | PEG 8000, Sodium Acetate, Imideazole pH 6.8, YbCl2, Bestatin, pH 8.0, LIQUID DIFFUSION, temperature 298K |
