1SQJ
Crystal Structure Analysis of Oligoxyloglucan reducing-end-specific cellobiohydrolase (OXG-RCBH)
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-18B |
| Synchrotron site | Photon Factory |
| Beamline | BL-18B |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2002-11-19 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.978, 0.9792, 0.9794, 0.9500 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 61.077, 146.051, 212.711 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.200 |
| R-factor | 0.21877 |
| Rwork | 0.217 |
| R-free | 0.25060 |
| Structure solution method | MAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.442 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.069 | 0.252 |
| Number of reflections | 294393 | |
| <I/σ(I)> | 9.5 | 3 |
| Completeness [%] | 87.5 | 70.9 |
| Redundancy | 3.5 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.8 | 293 | PEG 3000, PEG 400, pH 5.8, VAPOR DIFFUSION, temperature 293K |
