1SQI
Structural basis for inhibitor selectivity revealed by crystal structures of plant and mammalian 4-hydroxyphenylpyruvate dioxygenases
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-05-18 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 61.611, 107.481, 133.029 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.150 |
| R-factor | 0.222 |
| Rwork | 0.216 |
| R-free | 0.27100 |
| Structure solution method | MIR |
| RMSD bond length | 0.019 |
| RMSD bond angle | 0.041 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.250 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.068 | 0.248 |
| Number of reflections | 596675 | |
| Completeness [%] | 98.4 | 97.2 |
| Redundancy | 12.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | PEG2000, hexanediol, cobalt chloride, hepe, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
