1SQG
The crystal structure of the E. coli Fmu apoenzyme at 1.65 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.921 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.672, 48.695, 86.802 |
| Unit cell angles | 90.00, 108.65, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.650 |
| R-factor | 0.22461 |
| Rwork | 0.223 |
| R-free | 0.25721 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.316 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 31.400 |
| High resolution limit [Å] | 1.650 |
| Number of reflections | 56794 |
| <I/σ(I)> | 23.6 |
| Completeness [%] | 98.7 |
| Redundancy | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.4 | 293 | PEG4000, Tris-HCl, pH 8.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
