1SQF
The crystal structure of E. coli Fmu binary complex with S-Adenosylmethionine at 2.1 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-1 |
Synchrotron site | SSRL |
Beamline | BL9-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.921 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 57.407, 49.282, 86.920 |
Unit cell angles | 90.00, 109.47, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.100 |
R-factor | 0.23063 |
Rwork | 0.226 |
R-free | 0.27635 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.023 |
RMSD bond angle | 2.054 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.550 | 2.030 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 31286 | |
<I/σ(I)> | 18.9 | |
Completeness [%] | 99.0 | |
Redundancy | 6.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.4 | 293 | PEG4000, Tris-HCl, pH 8.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |