1SQB
Crystal Structure Analysis of Bovine Bc1 with Azoxystrobin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-D |
| Synchrotron site | APS |
| Beamline | 14-BM-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-02-20 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 153.554, 153.554, 596.393 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.690 |
| R-factor | 0.242 |
| Rwork | 0.241 |
| R-free | 0.28800 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1qcr |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.627 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.720 |
| High resolution limit [Å] | 2.690 | 2.690 |
| Number of reflections | 99537 | |
| Completeness [%] | 86.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.2 | 277 | 20mM ammonium acetate, 20% glycerol, 12% PEG4000, 0.5M KCl, 0.1% diheptanoyl-phosphatidylcholine, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 277K, pH 7.20 |
