1SQ6

Plasmodium falciparum homolog of Uridine phosphorylase/Purine nucleoside phosphorylase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2003-12-23
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9795
Spacegroup name	H 3 2
Unit cell lengths	95.087, 95.087, 134.786
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.260 - 2.400
R-factor	0.18403
Rwork	0.180
R-free	0.26501
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.269
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.260	2.330
High resolution limit [Å]	2.250	2.250
Number of reflections	8025
<I/σ(I)>	16	2.1
Completeness [%]	69.6	22

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	PEG3350, ammonium sulfate, MOPS, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K