1SQ6
Plasmodium falciparum homolog of Uridine phosphorylase/Purine nucleoside phosphorylase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-12-23 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 95.087, 95.087, 134.786 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.260 - 2.400 |
| R-factor | 0.18403 |
| Rwork | 0.180 |
| R-free | 0.26501 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.269 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.260 | 2.330 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Number of reflections | 8025 | |
| <I/σ(I)> | 16 | 2.1 |
| Completeness [%] | 69.6 | 22 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | PEG3350, ammonium sulfate, MOPS, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
