1SQ4
Crystal Structure of the Putative Glyoxylate Induced Protein from Pseudomonas aeruginosa, Northeast Structural Genomics Target PaR14
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-03-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 106.860, 106.860, 107.446 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.750 - 2.700 |
| R-factor | 0.262 |
| Rwork | 0.213 |
| R-free | 0.26200 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SnB |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.750 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.120 | 0.389 |
| Number of reflections | 32679 | |
| <I/σ(I)> | 21.9 | 7.9 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 12.8 | 11.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 20% PEG 3350 and 200mM potassium isothiocyanate, pH 7.00, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
