1SPX

Crystal Structure of Glucose Dehydrogenase of Caenorhabditis Elegans in the Apo-Form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2004-03-12
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.54
Spacegroup name	I 2 2 2
Unit cell lengths	64.682, 89.287, 108.153
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.540 - 2.100
R-factor	0.202
Rwork	0.202
R-free	0.25000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Starting model was a homology model from Swiss-Model based on PDB entries 1RWB and 1G6K
RMSD bond length	0.005
RMSD bond angle	1.200
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.180
High resolution limit [Å]	2.100	2.100
Rmerge	0.086	0.239
Number of reflections	17897
<I/σ(I)>	16.4
Completeness [%]	95.4	79.4
Redundancy	7.7	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	295	2M Ammonium Sulfate, 5% Isopropanol, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K