1SPI

CRYSTAL STRUCTURE OF SPINACH CHLOROPLAST FRUCTOSE-1,6-BISPHOSPHATASE AT 2.8 ANGSTROMS RESOLUTION

Experimental procedure

Spacegroup name	P 1 21 1
Unit cell lengths	76.100, 85.700, 105.800
Unit cell angles	90.00, 103.10, 90.00

Refinement procedure

Resolution	6.000 - 2.800
R-factor	0.203
Rwork	0.203
RMSD bond length	0.019
RMSD bond angle	2.250
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	15.000 *
High resolution limit [Å]	2.700 *
Rmerge	0.106 *
Number of reflections	29281
Completeness [%]	83.3

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	enzyme	10-15 (mg/ml)
2	1	2		80 (mM)
3	1	2	ammonium sulfate	0.3 (mM)
4	1	2	Na citrate	0.3 (mM)
5	1	2	PEG1450	2 (%(w/v))
6	1	2	PEG3350	2 (%(w/v))
7	1	2		4 (mM)
8	1	2	EDTA	0.2 (mM)
9	1	2	octylphenoxypoly(ethoxyethanol)	0.3 (mM)