1SNZ
Crystal structure of apo human galactose mutarotase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 275 |
| Detector technology | AREA DETECTOR |
| Collection date | 2004-02-02 |
| Detector | SIEMENS HI-STAR |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.700, 90.700, 70.000 |
| Unit cell angles | 90.00, 102.50, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.200 |
| R-factor | 0.174 |
| Rwork | 0.172 |
| R-free | 0.20100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1l7j |
| RMSD bond length | 0.013 |
| RMSD bond angle | 2.400 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | AMoRE |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.300 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 36670 | |
| <I/σ(I)> | 8.3 | 1.6 |
| Completeness [%] | 94.8 | 90.1 |
| Redundancy | 3.4 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | batch | 5 | 275 | PEG 8000, homopipes, sodium chloride, pH 5.0, batch, temperature 275K |
