1SNQ

PROTEIN STABILITY IN STAPHYLOCOCCAL NUCLEASE

Experimental procedure

Temperature [K]	277
Detector technology	IMAGE PLATE
Collection date	1993-02
Detector	RIGAKU RAXIS IIC
Spacegroup name	P 41
Unit cell lengths	49.180, 49.180, 63.510
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 1.950 *
R-factor	0.187
Rwork	0.187
R-free	0.27400
RMSD bond length	0.011 *
RMSD bond angle	1.602 *
Data reduction software	R-AXIS
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	9999.000 *
High resolution limit [Å]	1.950 *	1.950 *
Rmerge	0.047
Total number of observations	30773 *
Number of reflections	10604
Completeness [%]	94.5	90 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	8.15	4 *	Loll, P.J., (1989) Proteins: Struct.,Funct., Genet., 5, 183. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	drop	20 (mg/ml)
2	1	drop	potassium phosphate	10.5 (mM)
3	1	drop	MPD	17 (%(w/w))
4	1	drop		10 (mM)
5	1	drop	potassium citrate	20 (mM)
6	1	reservoir	potassium phosphate	10.5 (mM)
7	1	reservoir	MPD	20-30 (%(w/w))