1SNP

PROTEIN STABILITY IN STAPHYLOCOCCAL NUCLEASE

Experimental procedure

Temperature [K]	277
Detector technology	IMAGE PLATE
Collection date	1993-02
Detector	RIGAKU
Spacegroup name	P 41
Unit cell lengths	49.010, 49.010, 63.660
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 1.950
R-factor	0.184
Rwork	0.184
R-free	0.25500
RMSD bond length	0.010 *
RMSD bond angle	1.632 *
Data reduction software	R-AXIS
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	9999.000 *
High resolution limit [Å]	1.950 *	1.950 *
Rmerge	0.062
Total number of observations	36225 *
Number of reflections	11126
Completeness [%]	98.9	97.1 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	8.15	4 *	Loll, P.J., (1989) Proteins: Struct.,Funct., Genet., 5, 183. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	drop	potassium phosphate	10.5 (mM)
3	1	drop	MPD	17 (%(w/w))
4	1	drop		10 (mM)
5	1	drop	potassium citrate	20 (mM)
6	1	reservoir	potassium phosphate	10.5 (mM)
7	1	reservoir	MPD	20-30 (%(w/w))