1SNN
3,4-dihydroxy-2-butanone 4-phosphate synthase from Methanococcus jannaschii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-08-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.283, 1.2823 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.395, 69.366, 57.574 |
| Unit cell angles | 90.00, 93.58, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.550 |
| R-factor | 0.21 |
| Rwork | 0.200 |
| R-free | 0.23400 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1pvy |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.480 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.590 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.044 | 0.267 |
| Number of reflections | 53846 | |
| <I/σ(I)> | 17.5 | 2 |
| Completeness [%] | 0.9 | 92.8 |
| Redundancy | 2.4 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | PEG1000, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
