1SND

STAPHYLOCOCCAL NUCLEASE DIMER CONTAINING A DELETION OF RESIDUES 114-119 COMPLEXED WITH CALCIUM CHLORIDE AND THE COMPETITIVE INHIBITOR DEOXYTHYMIDINE-3',5'-DIPHOSPHATE

Experimental procedure

Detector technology	IMAGE PLATE
Collection date	1993-10
Detector	RIGAKU
Spacegroup name	P 41 21 2
Unit cell lengths	132.323, 132.323, 54.782
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 1.840
R-factor	0.176 *
Rwork	0.176
RMSD bond length	0.009
RMSD bond angle	0.031
Data reduction software	RIGAKU
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	6.000 *
High resolution limit [Å]	1.840 *
Rmerge	0.071
Total number of observations	157238 *
Number of reflections	34679
Completeness [%]	81.0
Redundancy	4.54

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6.8 *	4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	1.1 (mM)
2	1	drop	PIPES	1 (mM)
3	1	drop		50 (mM)
5	1	reservoir		10 (mM)
6	1	reservoir	MPD	40 (%(w/v))