1SNA
An Oligomeric Domain-Swapped Beta-Beta-Alpha Mini-Protein
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.978240, 0.977939, 0.949359 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 50.027, 46.868, 31.426 |
| Unit cell angles | 90.00, 98.61, 90.00 |
Refinement procedure
| Resolution | 24.220 - 1.500 |
| R-factor | 0.161 |
| Rwork | 0.161 |
| R-free | 0.19900 |
| Structure solution method | MAD |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.035 | 0.066 |
| Number of reflections | 17229 | |
| <I/σ(I)> | 33.8 | 7.7 |
| Completeness [%] | 76.0 | 24.9 |
| Redundancy | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 100 mM HEPES-Na, 10% v/v i-propanol, 20% w/v PEG 4000, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
