1SMV
PRIMARY STRUCTURE OF SESBANIA MOSAIC VIRUS COAT PROTEIN: ITS IMPLICATIONS TO THE ASSEMBLY AND ARCHITECTURE OF THE VIRUS
Experimental procedure
| Spacegroup name | R 3 |
| Unit cell lengths | 291.460, 291.460, 291.460 |
| Unit cell angles | 61.95, 61.95, 61.95 |
Refinement procedure
| Resolution | 10.000 - 3.000 |
| R-factor | 0.227 |
| Rwork | 0.227 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.776 |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 60.000 |
| High resolution limit [Å] | 2.900 |
| Rmerge | 0.280 * |
| Number of reflections | 347170 |
| Completeness [%] | 70.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | virus | 25-35 (mg/ml) | |
| 2 | 1 | drop | sodium acetate | 0.1 (M) | |
| 3 | 1 | drop | dithiothreitol | 10 (mM) | |
| 4 | 1 | drop | ammonium sulfate | 5-20 (%sat) | |
| 5 | 1 | reservoir | ammonium sulfate | 30-35 (%) | |
| 6 | 1 | reservoir | sodium acetate | 0.1 (M) | |
| 7 | 1 | reservoir | EDTA | 10 (mM) |
