1SMT

SMTB REPRESSOR FROM SYNECHOCOCCUS PCC7942

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	295
Detector technology	AREA DETECTOR
Collection date	1996-11
Detector	SIEMENS
Spacegroup name	P 1 21 1
Unit cell lengths	37.600, 70.900, 44.000
Unit cell angles	90.00, 95.70, 90.00

Refinement procedure

Resolution	100.000 - 2.200
R-factor	0.217
Rwork	0.217
R-free	0.25000
Structure solution method	MULTIPLE ISOMORPHOUS REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	19.900 *
Data reduction software	XENGEN
Data scaling software	XENGEN
Phasing software	X-PLOR (3.851)
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.000	2.340
High resolution limit [Å]	2.200	2.200
Rmerge	0.059 *	0.150 *
Total number of observations	18827 *
Number of reflections	10563
<I/σ(I)>	23.8199	3
Completeness [%]	90.0	69
Redundancy	1.8	1.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.1	23 *	used to seeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	40 (%sat)
2	1	reservoir	MES	100 (mM)