1SMS

Structure of the Ribonucleotide Reductase Rnr4 Homodimer from Saccharomyces cerevisiae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 5ID-B
Synchrotron site	APS
Beamline	5ID-B
Temperature [K]	113
Detector technology	CCD
Detector	MARRESEARCH
Wavelength(s)	1.000
Spacegroup name	P 61
Unit cell lengths	79.600, 79.600, 218.100
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	12.000 - 3.100
Rwork	0.266
R-free	0.30500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1JK0 subunit Y4
RMSD bond length	0.008
RMSD bond angle	1.400
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	12.000	3.150
High resolution limit [Å]	3.100	3.100
Number of reflections	239131
<I/σ(I)>	13.4	2.4
Completeness [%]	93.6	94.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	HEPES, PEG 1000, Ethanol, sodium chloride, EMTS, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K