1SMQ
Structure of the Ribonucleotide Reductase Rnr2 Homodimer from Saccharomyces cerevisiae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 5ID-B |
| Synchrotron site | APS |
| Beamline | 5ID-B |
| Temperature [K] | 113 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 79.670, 79.570, 88.050 |
| Unit cell angles | 83.47, 83.45, 70.79 |
Refinement procedure
| Resolution | 25.000 - 3.100 |
| Rwork | 0.272 |
| R-free | 0.30300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1JK0 subunit Y2 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.600 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 87.000 | 3.270 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Number of reflections | 168766 | |
| <I/σ(I)> | 7.2 | 2.5 |
| Completeness [%] | 94.6 | 96 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.8 | 287 | Succinate, PEG3350, lithium acetate, pH 4.8, VAPOR DIFFUSION, HANGING DROP, temperature 287K |
