1SMO
Crystal Structure of Human Triggering Receptor Expressed on Myeloid Cells 1 (TREM-1) at 1.47 .
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 118 |
Detector technology | CCD |
Collection date | 2003-06-07 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.0000, 0.9795, 0.9797, 0.9537 |
Spacegroup name | P 61 |
Unit cell lengths | 110.937, 110.937, 46.835 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 22.030 - 1.470 |
Rwork | 0.193 |
R-free | 0.20600 |
Structure solution method | MAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.545 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 22.030 | 1.510 |
High resolution limit [Å] | 1.470 | 1.470 |
Number of reflections | 55408 | |
<I/σ(I)> | 46.3 | 3 |
Completeness [%] | 96.7 | |
Redundancy | 4.2 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.3 | 277 | PEG 4000, Sodium Tartrate, pH 6.3, VAPOR DIFFUSION, SITTING DROP, temperature 277K, pH 6.30 |