1SMN

IDENTIFICATION OF THE SERRATIA ENDONUCLEASE DIMER: STRUCTURAL BASIS AND IMPLICATIONS FOR CATALYSIS

Experimental procedure

Detector technology	DIFFRACTOMETER
Collection date	1993-03-08
Detector	ENRAF-NONIUS FAST
Spacegroup name	P 21 21 2
Unit cell lengths	106.700, 74.500, 68.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 2.040
R-factor	0.168
Rwork	0.168
RMSD bond length	0.015
RMSD bond angle	25.500 *
Data reduction software	MADNES
Data scaling software	XSCALE
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
Completeness [%]	93.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microdialysis *	8.2 *		2 can be substituted with 40 mM Tris-HCl, pH 8.2, 2 mM MgCl, 1 M NaCl2; or 100 mM NaPO4, pH 6.0. *
1	Microdialysis *	8.2 *		2 can be substituted with 40 mM Tris-HCl, pH 8.2, 2 mM MgCl, 1 M NaCl2; or 100 mM NaPO4, pH 6.0. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	1-5 (mg/ml)
2	1	2	Tris-HCl	40 (mM)
3	1	2		2 (mM)
4	1	2		100 (mM)