1SMA
CRYSTAL STRUCTURE OF A MALTOGENIC AMYLASE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6B |
| Synchrotron site | Photon Factory |
| Beamline | BL-6B |
| Temperature [K] | 100 |
| Spacegroup name | P 61 |
| Unit cell lengths | 118.370, 118.370, 266.700 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 2.800 |
| R-factor | 0.209 |
| Rwork | 0.209 |
| R-free | 0.26700 |
| Structure solution method | OTHER |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.120 |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | |
| High resolution limit [Å] | 2.800 | |
| Rmerge | 0.040 | 0.120 * |
| Number of reflections | 40688 | |
| Completeness [%] | 94.8 | 87.3 * |
| Redundancy | 4.0 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 5.6 | 22 * | pH 5.60 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 8 (mg/ml) | |
| 10 | 1 | reservoir | ethanol | 4 (%(v/v)) | |
| 2 | 1 | drop | maleate | 16 (mM) | |
| 3 | 1 | drop | lithium sulfate | 0.1 (M) | |
| 4 | 1 | drop | ammonium sulfate | 0.06 (M) | |
| 5 | 1 | drop | sodium citrate | 0.02 (M) | |
| 6 | 1 | drop | ethanol | 0.8 (%(v/v)) | |
| 7 | 1 | reservoir | lithium sulfate | 0.5 (M) | |
| 8 | 1 | reservoir | ammonium sulfate | 0.3 (M) | |
| 9 | 1 | reservoir | sodium citrate | 0.1 (M) |
