Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.720, 108.980, 90.360 |
| Unit cell angles | 90.00, 95.57, 90.00 |
Refinement procedure
| Resolution | 8.000 * - 2.500 |
| R-factor | 0.209 |
| Rwork | 0.209 |
| R-free | 0.26500 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fnd poly-Ala |
| RMSD bond length | 0.007 * |
| RMSD bond angle | 1.600 * |
| Data scaling software | CCP4 ((AGROVATA) |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.580 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.094 | 0.319 |
| Total number of observations | 61664 * | |
| Number of reflections | 26895 | |
| Completeness [%] | 83.9 | 83.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 8 * | 20 * | TRIS/HCL, AMMONIUM SULFATE, VAPOR DIFFUSION |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | Tris-HCl | 0.1 (M) | pH8.5 |
| 2 | 1 | reservoir | ammonium sulfate | 1.9 (M) | |
| 3 | 1 | drop | protein | 15.0 (mg/ml) | |
| 4 | 1 | drop | Tris-HCl | 20 (mM) | pH8.0 |
