1SLY

COMPLEX OF THE 70-KDA SOLUBLE LYTIC TRANSGLYCOSYLASE WITH BULGECIN A

Experimental procedure

Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, Hamburg
Beamline	X11
Detector technology	IMAGE PLATE
Collection date	1993-10-20
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	80.310, 88.470, 133.060
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.800
R-factor	0.195 *
Rwork	0.185
R-free	0.26800
RMSD bond length	0.015
RMSD bond angle	21.400 *
Data reduction software	MOSFLM
Data scaling software	BIOMOL ((KBRANI))
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	22.000
High resolution limit [Å]	2.800
Rmerge	0.080
Number of reflections	23903
Completeness [%]	99.0
Redundancy	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.8 *		Rozeboom, H. J., (1990) J. Mol. Biol., 212, 557. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	potassium phosphate	10 (mM)
3	1	reservoir	ammonium sulfate	15-30 (%)
4	1	reservoir	sodium acetate	0.1 (M)