1SLW
RAT ANIONIC N143H, E151H TRYPSIN COMPLEXED TO A86H ECOTIN; NICKEL-BOUND
Experimental procedure
| Temperature [K] | 198 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-02-10 |
| Detector | RIGAKU |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 86.380, 56.230, 81.230 |
| Unit cell angles | 90.00, 92.81, 90.00 |
Refinement procedure
| Resolution | 6.000 - 2.000 |
| R-factor | 0.179 |
| Rwork | 0.179 |
| R-free | 0.25300 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 25.090 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | |
| High resolution limit [Å] | 2.000 | |
| Rmerge | 0.054 | |
| Total number of observations | 56521 * | |
| Number of reflections | 17281 | |
| Completeness [%] | 95.5 | 90.4 * |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 * | 18 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | trypsin and ecotin | 0.375 (mM) | |
| 10 | 1 | reservoir | Tris | 100 (mM) | |
| 11 | 1 | reservoir | 10 (mM) | ||
| 2 | 1 | drop | 300 (mM) | ||
| 3 | 1 | drop | Tris | 100 (mM) | |
| 4 | 1 | drop | PEG4000 | 18-22 (%) | |
| 5 | 1 | drop | 300 (mM) | ||
| 6 | 1 | drop | Tris | 100 (mM) | |
| 7 | 1 | drop | 10 (mM) | ||
| 8 | 1 | reservoir | PEG4000 | 18-22 (%) | |
| 9 | 1 | reservoir | 300 (mM) |
