1SLF

APOSTREPTAVIDIN, PH 5.6, TWO MOLECULES OF (SO4)2 BOUND AT THE BIOTIN BINDING SITE

Experimental procedure

Detector technology	AREA DETECTOR
Detector	SIEMENS
Spacegroup name	I 2 2 2
Unit cell lengths	95.430, 106.120, 47.540
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	7.500 - 1.740 *
R-factor	0.176
Rwork	0.176
R-free	0.20200
RMSD bond length	0.018
RMSD bond angle	27.800 *
Data reduction software	SADIE
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000 *
High resolution limit [Å]	1.740 *
Rmerge	0.073
Total number of observations	93159 *
Number of reflections	22185
Redundancy	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.6		pH 5.6

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	30-32 (mg/ml)
2	1	reservoir	potassium acetate	0.1 (M)
3	1	drop	protein	30 (mg/ml)