1SLD

STREPTAVIDIN, PH 7.5, BOUND TO CYCLIC DISULFIDE-BONDED PEPTIDE LIGAND AC-CHPQFC-NH2

Experimental procedure

Detector technology	AREA DETECTOR
Detector	SIEMENS
Spacegroup name	I 41 2 2
Unit cell lengths	59.250, 59.250, 178.350
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	7.500 - 2.000 *
R-factor	0.194
Rwork	0.194
R-free	0.22000
RMSD bond length	0.019
RMSD bond angle	28.300 *
Data reduction software	SADIE
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000 *
High resolution limit [Å]	1.920 *
Rmerge	0.091
Total number of observations	52950 *
Number of reflections	11347
Completeness [%]	39.0
Redundancy	4.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	4 *		pH 7.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	peptide	15 (mg/ml)