1SKV

Crystal Structure of D-63 from Sulfolobus Spindle Virus 1

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-D
Synchrotron site	APS
Beamline	14-BM-D
Temperature [K]	100
Detector technology	CCD
Collection date	2002-08-16
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9778, 0.9787, 0.9789
Spacegroup name	P 62
Unit cell lengths	110.650, 110.650, 47.450
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	15.000 - 2.600
R-factor	0.2245
Rwork	0.222
R-free	0.26950
Structure solution method	MAD
RMSD bond length	0.016
RMSD bond angle	1.534
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	REFMAC (5.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.700
High resolution limit [Å]	2.600	2.600
Rmerge	0.043	0.212
Number of reflections	10095
<I/σ(I)>	42	9
Completeness [%]	98.7	99.5
Redundancy	8.6	9.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	291	Ammonium Sulfate, ammonium acetate, PEG 4000, NaCl, Tris, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K