1SKQ
The crystal structure of Sulfolobus solfataricus elongation factor 1-alpha in complex with magnesium and GDP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-11-30 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.99 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 61.950, 113.830, 81.080 |
Unit cell angles | 90.00, 90.10, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.800 |
R-factor | 0.223 |
Rwork | 0.219 |
R-free | 0.25500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1jny |
RMSD bond length | 0.019 |
RMSD bond angle | 2.000 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.035 | 0.343 |
Number of reflections | 98914 | |
Completeness [%] | 94.3 | 80 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | microbatch under oil | 5.6 | 277 | PEG4000, isopropanol, sodium citrate, pH 5.6, microbatch under oil, temperature 277K |