1SKQ
The crystal structure of Sulfolobus solfataricus elongation factor 1-alpha in complex with magnesium and GDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-11-30 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.99 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.950, 113.830, 81.080 |
| Unit cell angles | 90.00, 90.10, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.800 |
| R-factor | 0.223 |
| Rwork | 0.219 |
| R-free | 0.25500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jny |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.000 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.035 | 0.343 |
| Number of reflections | 98914 | |
| Completeness [%] | 94.3 | 80 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | microbatch under oil | 5.6 | 277 | PEG4000, isopropanol, sodium citrate, pH 5.6, microbatch under oil, temperature 277K |
