1SKN

THE BINDING DOMAIN OF SKN-1 IN COMPLEX WITH DNA: A NEW DNA-BINDING MOTIF

Experimental procedure

Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1997-03-15
Detector	MARRESEARCH
Spacegroup name	C 2 2 21
Unit cell lengths	82.130, 93.390, 53.450
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.500
R-factor	0.225 *
Rwork	0.220
R-free	0.33700
Structure solution method	MIRAS
RMSD bond length	0.017
RMSD bond angle	21.100 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.570
High resolution limit [Å]	2.500	2.500
Rmerge	0.038	0.116
Number of reflections	7202
Completeness [%]	94.8	100
Redundancy	6.9	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	277	PROTEIN WAS CRYSTALLIZED FROM 8% PEG 1000 50 MM BIS-TRIS, PH 6.0, 100-150 MM LICL, 2MM LDAO., VAPOR DIFFUSION, HANGING DROP, temperature 277.00K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	PEG 1000
2	1	1	LICL
3	1	1	BIS-TRIS
4	1	1	LDAO
5	1	2	PEG 1000
6	1	2	LICL
7	1	2	BIS-TRIS

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	0.5 (mM)
2	1	drop	5'-15mer	0.55 (mM)
3	1	drop	3'-15mer	0.55 (mM)
4	1	drop		50 (mM)
5	1	drop	N-lauryl-N,N-dimethylamine-N-oxide	2 (mM)
6	1	reservoir	PEG1000	7-8 (%)
7	1	reservoir		150 (mM)
8	1	reservoir	Bis-Tris	50 (mM)