1SK9
Crystallographic snapshots of Aspergillus fumigatus phytase revealing its enzymatic dynamics
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-10-21 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 134.209, 70.063, 47.087 |
| Unit cell angles | 90.00, 93.63, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.640 |
| R-factor | 0.15483 |
| Rwork | 0.153 |
| R-free | 0.18760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.670 |
| High resolution limit [Å] | 1.640 | 1.640 |
| Number of reflections | 52974 | |
| Completeness [%] | 99.3 | 94 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 300 | PEG 4000, magnesium chloride hexahydrate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 300K |
