1SK0
ARSENATE REDUCTASE R60A MUTANT +0.4M ARSENITE FROM E. COLI
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-11-09 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 86.560, 86.560, 115.990 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.800 |
| Rwork | 0.183 |
| R-free | 0.23850 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | NATIVE STRUCTURE |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.024 |
| Data reduction software | X-GEN |
| Data scaling software | X-GEN |
| Phasing software | SHELX |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.847 |
| High resolution limit [Å] | 1.788 | 1.788 |
| Rmerge | 0.142 | 0.526 |
| Number of reflections | 24478 | |
| <I/σ(I)> | 16.2 | 2.936 |
| Completeness [%] | 98.2 | 89.08 |
| Redundancy | 17.5 | 21.36 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.8 | 278 | 50% saturated Cesium Sulfate, 100mM sodium acetate,5mM DTT, pH 4.80, VAPOR DIFFUSION, HANGING DROP, temperature 278K |
