1SJM
Nitrite bound copper containing nitrite reductase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-02-27 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.08 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 61.600, 102.450, 145.980 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.850 - 1.400 |
| R-factor | 0.11465 |
| Rwork | 0.113 |
| R-free | 0.13846 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1j9q |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.398 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.450 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.041 | 0.121 |
| Number of reflections | 1173705 | |
| <I/σ(I)> | 21 | |
| Completeness [%] | 97.3 | 93.4 |
| Redundancy | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 298 | PEG 4000, sodium acetate, VAPOR DIFFUSION, HANGING DROP, temperature 321K |
