1SJ7

Crystal Structure of Talin Rod 482-655

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9793
Spacegroup name	P 32 2 1
Unit cell lengths	95.662, 95.662, 115.215
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.163 - 2.500
Rwork	0.241
R-free	0.28619
Structure solution method	SAD
RMSD bond length	0.026
RMSD bond angle	2.552
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.590
High resolution limit [Å]	2.500	2.500
Rmerge	0.059	0.312
Number of reflections	21513
<I/σ(I)>	28.05	3.576
Completeness [%]	99.8	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	298	30% PEG 2000 MME, 0.1M Ammonium Acetate, 0.2M Ammonium Sulfate, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 298K