1SIB

REFINED CRYSTAL STRUCTURES OF SUBTILISIN NOVO IN COMPLEX WITH WILD-TYPE AND TWO MUTANT EGLINS. COMPARISON WITH OTHER SERINE PROTEINASE INHIBITOR COMPLEXES

Experimental procedure

Spacegroup name	P 31 2 1
Unit cell lengths	84.900, 84.900, 89.100
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	5.000 - 2.400
R-factor	0.159
RMSD bond length	0.011
RMSD bond angle	0.048
Refinement software	PROLSQ

Data quality characteristics

	Overall
High resolution limit [Å]	2.400 *
Rmerge	0.097 *
Total number of observations	74306 *
Number of reflections	24538 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	60 (mg/ml)
2	1	reservoir	PEG4000	10-15 (%(w/v))
3	1	reservoir	potassium phosphate	0.1 (M)