1SI1
Crystal Structure of Mannheimia haemolytica Ferric iron-Binding Protein A in an open conformation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.3 |
| Synchrotron site | ALS |
| Beamline | 5.0.3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-07-20 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.031, 48.191, 77.362 |
| Unit cell angles | 90.00, 100.92, 90.00 |
Refinement procedure
| Resolution | 76.700 - 1.450 |
| R-factor | 0.1722 |
| Rwork | 0.171 |
| R-free | 0.19132 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1q35 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.176 |
| Data reduction software | HKL-2000 |
| Data scaling software | CCP4 ((TRUNCATE)) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 76.700 | 1.500 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Number of reflections | 55606 | |
| Completeness [%] | 95.0 | 98.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.2 | 277 | PEG 3350, sodium citrate, pH 8.2, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
